Rotation Project

During my first semester, I rotated through the Golos lab for 5 weeks. My project during that time was Listeria infection of a tissue culture using two different colored fluorescenly tagged bacterium. The purpose of the study was to test the efficiency of a pre-immunflourescent tagged strain, which would save processing time and reagents for tissue samples during animal experiments. I prepared and performed tissue cell cultures and bacterial cultures. I then inoculated the tissue samples with either the untagged or pre-tagged listeria, allowed the bacteria to infect the cells, and then the tissues are fixed with antibodies for histology analysis. The histology slides were then examined under fluorescent microscopy to image the bacteria. The two different colored bacteria can be seen below. The bright red or teal small rods are the Listeria bacteria, with the nuclei of the cells in navy and the cell membranes in green.